Characterization of a tobacco Bright Yellow 2 cell line expressing the tetracycline repressor at a high level for strict regulation of transgene expression.
Identifieur interne : 000870 ( Main/Exploration ); précédent : 000869; suivant : 000871Characterization of a tobacco Bright Yellow 2 cell line expressing the tetracycline repressor at a high level for strict regulation of transgene expression.
Auteurs : K M David [France] ; C. Perrot-RechenmannSource :
- Plant physiology [ 0032-0889 ] ; 2001.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Cinétique (MeSH), Glucuronidase (analyse), Glucuronidase (génétique), Lignée cellulaire (MeSH), Protéines de répression (génétique), Protéines recombinantes (analyse), Protéines recombinantes (biosynthèse), Tabac (cytologie), Tabac (génétique), Transfection (MeSH), Tétracycline (pharmacologie), Végétaux toxiques (MeSH).
- MESH :
- analyse : Glucuronidase, Protéines recombinantes.
- biosynthèse : Protéines recombinantes.
- cytologie : Tabac.
- génétique : Agrobacterium tumefaciens, Glucuronidase, Protéines de répression, Tabac.
- pharmacologie : Tétracycline.
- Cinétique, Lignée cellulaire, Transfection, Végétaux toxiques.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Cell Line (MeSH), Glucuronidase (analysis), Glucuronidase (genetics), Kinetics (MeSH), Plants, Toxic (MeSH), Recombinant Proteins (analysis), Recombinant Proteins (biosynthesis), Repressor Proteins (genetics), Tetracycline (pharmacology), Tobacco (cytology), Tobacco (genetics), Transfection (MeSH).
- MESH :
- chemical , analysis : Glucuronidase, Recombinant Proteins.
- chemical , biosynthesis : Recombinant Proteins.
- cytology : Tobacco.
- genetics : Agrobacterium tumefaciens, Glucuronidase, Repressor Proteins, Tobacco.
- chemical , pharmacology : Tetracycline.
- Cell Line, Kinetics, Plants, Toxic, Transfection.
Abstract
Manipulating the expression of a transgene in transient and stable transformed cells is a requirement for many functional analyses. We have investigated the use of the tetracycline-dependent gene expression system developed by Gatz et al. (1992) in tobacco (Nicotiana tabacum L. cv Bright Yellow 2 [BY2]) cells, the most widely used plant cell culture. We have selected a BY2 cell line, named BY2-tetracycline repressor (tetR) 17, which expresses the tetR at a high level, and have evaluated the capacity of this cell line to suppress the expression of a green fluorescent protein reporter gene under the control of the "Triple-Op" promoter in the absence of tetracycline in a large number of independent transformants. The ability to induce the expression of green fluorescent protein after treatment by anhydrotetracycline in the same transformants was also analyzed. BY2-tetR17 cells were demonstrated to be excellent recipient cells for recovery of clonal cell lines with a highly controlled regulation of the introduced transgene.
DOI: 10.1104/pp.125.4.1548
PubMed: 11299335
PubMed Central: PMC1539379
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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We have investigated the use of the tetracycline-dependent gene expression system developed by Gatz et al. (1992) in tobacco (Nicotiana tabacum L. cv Bright Yellow 2 [BY2]) cells, the most widely used plant cell culture. We have selected a BY2 cell line, named BY2-tetracycline repressor (tetR) 17, which expresses the tetR at a high level, and have evaluated the capacity of this cell line to suppress the expression of a green fluorescent protein reporter gene under the control of the "Triple-Op" promoter in the absence of tetracycline in a large number of independent transformants. The ability to induce the expression of green fluorescent protein after treatment by anhydrotetracycline in the same transformants was also analyzed. BY2-tetR17 cells were demonstrated to be excellent recipient cells for recovery of clonal cell lines with a highly controlled regulation of the introduced transgene.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11299335</PMID><DateCompleted><Year>2001</Year><Month>08</Month><Day>02</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>125</Volume><Issue>4</Issue><PubDate><Year>2001</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Characterization of a tobacco Bright Yellow 2 cell line expressing the tetracycline repressor at a high level for strict regulation of transgene expression.</ArticleTitle><Pagination><MedlinePgn>1548-53</MedlinePgn></Pagination><Abstract><AbstractText>Manipulating the expression of a transgene in transient and stable transformed cells is a requirement for many functional analyses. We have investigated the use of the tetracycline-dependent gene expression system developed by Gatz et al. (1992) in tobacco (Nicotiana tabacum L. cv Bright Yellow 2 [BY2]) cells, the most widely used plant cell culture. We have selected a BY2 cell line, named BY2-tetracycline repressor (tetR) 17, which expresses the tetR at a high level, and have evaluated the capacity of this cell line to suppress the expression of a green fluorescent protein reporter gene under the control of the "Triple-Op" promoter in the absence of tetracycline in a large number of independent transformants. The ability to induce the expression of green fluorescent protein after treatment by anhydrotetracycline in the same transformants was also analyzed. 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Perrot-Rechenmann</name></noCountry><country name="France"><region name="Île-de-France"><name sortKey="David, K M" sort="David, K M" uniqKey="David K" first="K M" last="David">K M David</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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